By4742基因型
Web感受态细胞操作方法. 1. 取BY4741感受态细胞置于冰浴中,如需分装可将刚融化细胞悬液分装到无菌预冷的离心管中,置于冰浴中。. 注意:一次转化BY4741感受态细胞的建议用 …
By4742基因型
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WebBY4742感受态细胞. 产品储存:-80 ℃保存,一年有效。. 1. 取 100 µL 冰上融化的感受态细胞,依次加入预冷的目的质粒 2-5 µg,Carrier DNA (95-100 ℃,5min,快 速冰浴,重复一次)10 µL,PEG/LiAc 500 µL 并吸打几次混匀,30℃水浴 30 min (15 min 时翻转 6-8 次混匀)。. 2. 将离心管 ... Webv Important Information Contents 20 µg of pYD1, lyophilized in TE, pH 8 EBY100 glycerol stock, 0.5 ml 2 µg pYD1 Forward sequencing primer, lyophilized 2 µg pYD1 Reverse sequencing primer, lyophilized Shipping/Storage The pYD1 Vector Kit is shipped on dry ice. Upon receipt: • Store the glycerol stock at -80°C • Store the lyophilized plasmid and …
Web产品描述. BY4741菌株是来源于S288C的一组缺失菌株成员之一,这些菌株通过敲除其常用选择性标记基因来最小化或去除与对应载体上常用的选择性标记基因的同源性,同时不 … WebBY4742为酿酒酵母菌株。 基因型: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0
http://euroscarf.de/plasmid_details.php?accno=Y20000 WebJun 15, 2011 · 关注. 不一样,S288C是含有酿酒酵母全部基因组的菌株;BY4741是敲除了一些利用某些氨基酸的菌株,BY4741含有酵母基因组96%的基因。. 本回答由提问者推荐. 7. 评论 (3) 分享. 举报. 饲料所丁宏标. 2011-06-15 · TA获得超过240个赞.
WebBY4741 was used as a parent strain for the international systematic Saccharomyces cerevisiae gene disruption project. Brachmann CB, et al. (1998) Designer deletion …
WebFeb 27, 2024 · As in S288c, this strain as well as haploid derivatives BY4741, and BY4742 have allelic variants of MIP1, SAL1 and CAT5 and these polymorphisms, described in the respective locus history notes for these genes (MIP1, SAL1 and CAT5) all contribute to the high observed petite frequency. taste of home recipes chiliWeb[0034] 所述ρΤ0Ρ0+ΜΑΤα质粒的模板为酿酒酵母BY4742基因组。 [0035] 所述ρΤΟΡΟ+MATa质粒的模板为酿酒酵母ΒΥ4741基因组。 [0036]本发明所述转换酵母交配型的方法所述转化体系中所述质粒DNA组成为每150yL 中含50ng guide-RNA质粒和5yL MAT基因表达盒质粒,余量为水。 taste of home recipes chili con carnehttp://www.bnbiotech.com/beinuobio-Products-11339967/ taste of home recipes corned beefWebBY4742,W303-1A,FY837,YS58. 1、长期保存的储存菌株以冷冻干燥保藏为主,特殊要求的菌种可用其它方法进行保存,保存期可长达几年至几十年。. 2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主,在0℃以下保存,保存期为3-6个月。. 3、应用菌株 … taste of home recipes cherry barsWeb如BY4742基因型:MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0,我知道是his3缺失,但“1”又代表什么呢,ura3 和ura3Δ0有什么区别呢? 展开 我来答 taste of home recipes dipshttp://muchong.com/t-10952789-1 taste of home recipes basic homemade breadhttp://www.zomanbio.com/shengwu_info.php?nid=1715 taste of home recipes chocolate cake