Hygromycin phosphotransferase hpt gene

Author: pznh

August undefined, 2024

http://jopr.mpob.gov.my/genetic-transformation-of-oil-palmbased-on-selection-with-hygromycin/ Web列表数据仅在虚线下方。全文数据即将推出。

Analisis Segregasi Gen hpt (Hygromisin Phosphotransferase) pada …

WebGene: aph4 (hpt) Gene Source: Escherichia coli Product: hygromycin-B phosphotransferase (hph) enzyme Function: allows selection for resistance to the … Web12 aug. 2013 · Hygromycin- and kanamycin-resistant transformants contained hygromycin B phosphotransferase- and neomycin phosphotransferasehybridizing restriction … the clorox company rogers ar

Selectable marker genes in transgenic plants: applications ...

Web在大肠杆菌中进行了HPT蛋白质的重组表达,利用纯化的HPT蛋白质制备了其特异性的单克隆抗体,采用免疫印迹学方法对转基因水稻4021中HPT蛋白质的表达情况进行了检测,该方 … WebThe objective of this study was to determine the efficacy of three commonly used Agrobacterium strains in genetic transformation of the microalga Chlamydomonas reinhardtii. Three different strains of Agrobacterium tumefaciens (EHA 101, EHA 105, LBA WebFour OsITPK6 mutant lines were generated by targeted mutagenesis of the gene’s first exon using the CRISPR/Cas9 method, one (ositpk6_1) with a 6-bp in-frame deletion, and other three with frameshift mutations ... In total, we obtained 23 hygromycin phosphotransferase (HPT)-positive T 0 plants transformed with the CRISPR/Cas9 … the clorox company university park il

Hygromycin Resistance Gene Cassettes for Vector Construction and

Gene transfer with subsequent removal of the selection gene from …

WebNIKSON HALOMOAN. Segregation Analysis of hpt (hygromycin phosphotransferase) Gene in T0 Generation Transgenic Tobacco Plants. Supervised by SUHARSONO and UTUT … WebA transformation system for the ringworm-producing dermatophyte Trichophyton mentagrophytes has been developed. The system employs the plasmid pHIS, which … the close 7 level sellingWebEmbryogenic calli induced after 20 days of highly regenerating rice cultivar BRRI dhan29 and Agrobacterium strain GV 3101 was transformed with binary vector pCAMBIA 1303 … the clorox company morrisville nc address

"Web5 jun. 2008 · Hundreds of hygromycin-resistant plants per sporangium were obtained by co-cultivation of immature thalli with Agrobacterium carrying the binary vector that … " - Hygromycin phosphotransferase hpt gene

Hygromycin phosphotransferase hpt gene

Hygromycin B-induced cell death is partly mediated by reactive …

WebRui Zhang is an academic researcher. The author has contributed to research in topic(s): Cotyledon & Transformation (genetics). The author has an hindex of 1, co-authored 1 publication(s) receiving 10 citation(s). WebImmunogen: ABI30072.1 Synonyms: HPT Background: The hygromycin phosphotransferase (denoted hpt, hph or aphIV) gene was originally derived from …

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Web21 jan. 2024 · The CRISPR/Cas9 technique for rice genome engineering is gaining momentum and requires a precise gene delivery system. For rice and other crop plants, … Web24 sep. 2011 · Of three major selectable genes, the bialaphos resistance (bar) gene and the hygromycin phosphotransferase (hpt) allowed effective selection of transformed cells …

WebA total of 3.46 x 10(8) protoplasts isolated from 17 independent transgenic Arabidopsis lines harbouring the defective chimeric hpt gene were transformed via direct gene … WebSequencing of plasmid DNA extracted from this strain indicated that it contains defesin gene (AFP), an antifungal protein-coding gene, under the control of a CaMV 35S promoter …

Web1 feb. 2009 · Conclusion HPT protein does not have any safety problems to human health. Key words: Hygromycin B phosphotransferase; Selectable marker; Acute toxicity; … Web1) hygromycin phosphotransferase (hpt) 潮霉素磷酸转移酶基因 (hpt) 2) hygB 潮霉素磷酸转移酶基因 1. We transformed the nematode-trapping fungus Arthrobotrys vermicola …

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WebGen hpt difusikan dengan gen MmCu-Zn-SOD, maka tanaman T0 juga mengandung gen MmCu-Zn-SOD. Kata kunci : segregasi, higromisin, analisis khi-kuadrat. ABSTRACT . … the close airport hotel heathrowWebFatty acid (FA) composition determines the quality of oil from oilseed crops, and thus is a major target for genetic improvement. FAD2 (Fatty acid dehydrogenase 2) and FAE1 (fatty acid elongase 1) are critical FA synthetic genes, and have been the focus of genetic manipulation to alter fatty acid composition in oilseed plants. In this study, to improve the … the close burgess hillWeb5 feb. 2004 · The E. coli gene aphIV (hph, hpt), coding for hygromycin B phosphotransferase (HPT, E.C. 2.7.1.119), confers resistance on bacteria, fungi, … the close harborne shipwaysWebHPT / Anti-HPT Antibody. Starting at $89.00. Add to Wish List. Immunogen: ABI30072.1. Synonyms: HPT. Background: The hygromycin phosphotransferase (denoted hpt, … the close function takes one argumentWebTransformation and inheritance of a hygromycin phosphotransferase gene in maize plants. Embryogenic maize (Zea mays L.) callus cultures were transformed by … the close corporation act of 1984Web1 dec. 1991 · Abstract Abstract A general method of gene transfer that does not leave behind a selectable marker in the host genome is described. A luciferase gene was … the close cheltenhamWeb2 sep. 2024 · The hygromycin phosphotransferase (HPT) gene as a selective marker is normally used in screening tests as a first step in detecting and quantifying genetically … the close full show