Phosphinothricin acetyltransferase gene

WebAbstract. Insertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower … WebSKU: GENE-BT-CP4 Category: Foody Safety Assays Tag: plantExtract. Related products. Ovine DNA Real Time PCR Detection Kit. Read more. cp4Epsps Gene Rapid Test Kit. ... phosphinothricin-N-acetyltransferase Test pAT/cp4 Epsps combo Test. Scroll to top. This site uses cookies. By continuing to browse the site, you are agreeing to our use of ...

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WebIt codes for phosphinothricin acetyl transferase (PAT) proteins of 183 amino acids and shows 85% DNA sequence homology with another marker gene pat isolated from Streptomyces viridochromogenes. WebMar 18, 2016 · Gene: BASTA selectable marker from Streptomyces hygrosopicus- phosphinothricin acetyltransferase gene to confer BASTA resistance as a selectable marker Promoter: 35S promoter from Cauliflower mosaic caulimovirus – CaMV 35S constitutive promoter to drive expression of GUS reporter gene rayleigh rebels https://kozayalitim.com

phosphinothricine acétyltransférase - Translation into English ...

WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... WebJun 15, 1992 · Cloning of a phosphinothricin N- acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Streptomyces lividans and Escherichia coli Gene (1988) P.G. Reddy et al. Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinobacter calcoaceticus RAG-1 Gene … WebStrauch, E.; Wohlleben, W.; Puhler, A.: Cloning of a phosphinothricin Nacetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli. Gene, 63, 65–74 (1988) CrossRef CAS PubMed Google Scholar rayleigh recompensa

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Category:Non-specific activities of the major herbicide-resistance gene BAR …

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Phosphinothricin acetyltransferase gene

Assessment of conditions affecting Agrobacterium-mediated …

WebSKU: GENE-PHY Category: Foody Safety Assays Tag: plantExtract. Related products. Microalbuminuriasemi-auantitative Test. Read more. Mouse DNA Real Time PCR Dection Kit. Read more. phosphinothricin-N-acetyltransferase Test. Read more. Round up Ready cp4 Epsps protein (HerbicideResistant) ultra sensitive Test. WebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector …

Phosphinothricin acetyltransferase gene

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WebApr 3, 2024 · Resveratrol-enriched rice (RR) includes the stilbene synthase gene for resveratrol synthesis and the phosphinothricin-N-acetyltransferase gene for glufosinate tolerance.To investigate unintended effects resulting from RR’s genetically modified chemical composition, 56 polar and nonpolar secondary metabolites were analyzed with …

WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … WebDroege, W.; Broer, I.; Puehler, A.: Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide l-phosphinothricin (glufosinate) …

WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. Web10.1007/BF00201636 Abstract L-Phosphinothricin (L-Pt)-resistant plants were constructed by introducing a modified phosphinothricin-N-acetyl-transferase gene (pat) via …

Webmechanism of Agrobacterium-mediated gene transfer has been widely utilized to genetically modify mono-cots and dicots (Hansen and Wright, 1999; Fukuoka et al., 2000). Agrobacterium tumefaciensis a soil-borne pathogen that has the capacity to infect plants and transfer a segment of DNA (T-DNA) from its tumor-inducing (Ti) plasmid to the plant …

WebApr 1, 2024 · Only few glufosinate-tolerant genes, such as phosphinothricin acetyltransferase ( PAT) and bialaphos resistance ( bar) identified from Streptomyces, are currently available for developing genetically modified rice in agricultural application. rayleigh recycling castle roadWebJan 1, 1995 · Phosphinothricin is an inhibitor of glutamine synthetase, which plays a central role in the assimilation of ammonia and in the regulation of the nitrogen metabolism in plants. 9, 10 It is the only enzyme that detoxifies ammonia produced during nitrate reduction, photorespiration, and amino acid degradation in plant cells. rayleigh recycling centre opening timesWebFeb 1, 2014 · The genes encoding for bialaphos resistance (phosphinothricin acetyltransferase), originally cloned from bialaphos producing streptomycetes [ 3, 56, 59, 63 ], have been used as selection markers in plant biology and engineering [ 11 ]. simple while loop program in pythonWebDownload scientific diagram Schematic structure of the T-DNA region of the binary vector pCAMBIA3301. LB, left border; RB, right border; bar, phosphinothricin acetyltransferase gene; gus-int, β ... rayleigh recycling centreWebphosphinothricin-N-acetyltransferase Test. SKU: GENE-PHOS-NAT Category: Foody Safety Assays Tag: plantExtract. Related products. urinalysis test … simple whiskey glassesWebIn this study, we identified a tau-like glutathione transferase family gene from soybean named GmGSTU23. ... GmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance gene (phosphinothricin N-acetyltransferase, PAT) ... simple whipped body butter recipeWebPhosphinothricin N-acetyltransferase. Gene. bar. Status. UniProtKB reviewed (Swiss-Prot) Organism. Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) Amino acids. 171. Protein existence. Evidence at protein level. Annotation score. 3/5. simple whip cream recipe